The aim of this study was to determine the frequency of Campylobacter species in stool specimens that were sent to the microbiology laboratory for routine stool culture because of gastroenteritis. Totally 882 stool samples were taken from Trakya University Medical Faculty clinics and out-patients clinics in 14 months (01 August 2001-30 September 2002). For identification of Campylobacter species, the samples were inoculated onto charcoal cefaperazon deoxycholate agar (modified-CCDA) and incubated at 42° C for 48 hours in microaerophilic atmosphere. Campylobacter species were differentiated according to Gram staining, typical motility in phase contrast microscope, oxidase and catalase reactions, and hippurate hydrolysation and nitrate reduction. In 113 (13 %) of 882 stool cultures, gastrointestinal pathogens were isolated. The most prevalent aetiological agent was Salmonella spp. (46 isolates, 41% of positive stool cultures, 5% of the total stool specimens), followed by Shigella spp. (36 isolates, 32% of positive stool cultures, 4% of the total stool specimens) and Campylobacter spp. (31 isolates, 27% of positive stool cultures, 4% of the total stool specimens). Salmonella enteritidis was isolated in 36 (78%) and S. typhimurium in 10 (22 %) of the 46 Salmonella isolates. Shigella flexneri was isolated in 18 (50 %), S. sonnei in 14 (39 %), S. boydii in 4 (11 %) of the 36 Shigella isolates. Campylobacter jejuni was isolated in 25 (81 %), C. coli in 6 (19 %) of the 31 Campylobacter isolates. Results of the antibiotic susceptibility tests of C. jejuni strains by agar dilution method revealed that they were all resistant to trimethoprim-sulfamethoxazole but susceptible to gentamicin and tetracycline. Results of other antibiotics were as following: 92 % susceptible to erythromycin, azithromycin and ciprofloxacin; 80% sensitive to ampicillin-sulbactam, 68 % susceptible to ampicillin. Campylobacter coli strains were susceptible to ciprofloxacin, gentamicin and tetracycline but resistant to trimethoprim-sulfamethoxazole; 83 % of them were susceptible to ampicillin and ampicillin-sulbactam and 67 % were susceptible to erythromycin and azithromycin. Data presented in this study indicate that Campylobacter species were one of the most common causes of acute gastroenteritidis in Edirne and were generally isolated from stool samples in summer and no significant difference was found between age groups. Routine stool cultures should include Campylobacter isolation. Macrolide group antibiotics can be used as the first choice in the treatment of Campylobacter gastroenteritis.