The purpose of this study was to evaluate the nosocomial infections developing in patients most of whom had spinal cord injury at the Turkish Army Force Rehabilitation and Care Center between January 1, 2002, and December 31, 2003. Totally 458 microorganisms were isolated from 380 hospital infections in 294 of 3746 patients in 2002 while totally 326 microorganisms were isolated in 283 hospital infections observed in 241 of 3966 patients in 2003. The rate of hospital infections in 2002 and 2003 were found as 10.14% and 7.14%, respectively. When the ratio of hospitalized patients with nosocomial infection to the clinics was evaluated, it was observed that acute care clinic had the highest ratios in both years (37.96% and 35.98%, respectively). In both years, the most frequent type of nosocomial infection was found to be asymptomatic bacteriuria (55.26% and 57.59%, respectively) and the most frequently isolated nosocomial microorganism Escherichia coli (33.41 % and 40.49%, respectively). Against Enterobacteriaceae, piperacillin/tazobactam and imipenem were found as the most effective, ampicillin and trimetopim/ sulfametokazol as the least effective agents. While piperacillin/tazobactam and imipenem were found to be the most effective agents against Gram-negative nonfermentative bacilli, trimethoprim/sulfamethoxazole and aztreonam were found to be the least effective agents. In addition, the isolation rates of methicillin-resistant Staphylococcus aureus as cause of nosocomial infection were 3.74% and 4.60% in 2002 and 2003, respectively. It is concluded that in the prevention of nosocomial infections controlled use of antibiotics, active surveillance, isolation and training of the personal are important issues.