2005, Cilt 19, Sayı 4, Sayfa(lar) 445-451 |
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EVALUATION OF MICRODILUTION METHOD USING HIGH RESOLUTION MEDIUM FOR ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS |
M. Cem ERGON, Mine YÜCESOY |
Dokuz Eylül Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İzmir |
Keywords: Yeast, antifungal susceptibility testing, RPMI 1640, HR medium |
The current study was undertaken to evaluate the microdilution method using high resolution medium for the susceptibility testing of yeasts. A total of 194 yeast isolates (78 Candida albicans, 42 C. glabrata, 33 C. tropicalis, 18 C. parapsilosis, 11 C. krusei, three C. guilliermondii and nine Trichosporon spp.) that were recovered from various clinical specimens were included. The susceptibilities of the isolates against amphotericin B, fluconazole and ketoconazole were determined by microdilution methods using RPMI 1640 medium buffered with MOPS and high resolution medium buffered with phosphate buffer. Overall results of both methods were considered to be in agreement when the MIC values were within ±2 fold dilutions. The agreement rates of MIC values of amphotericin B, fluconazole and ketoconazole were 84%, 62% and 81%, respectively. The agreement rates for the susceptibility categories of amphotericin B and fluconazole were 95% and 70%. As a result, it is concluded that high resolution medium can be used in the microdilution method when determining the susceptibilities especially susceptibility categories of Candida and Trichosporon spp. against amphotericin B, fluconazole and ketoconazole.
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