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2007, Cilt 21, Sayı 3, Sayfa(lar) 105-115
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PLASMID PROFILES AND RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS OF SALMONELLA ENTERICA SEROTYPE TYPHIMURIUM ISOLATED IN TÜRKİYE
Birsel ERDEM, Alper TEKELİ, Fikret ŞAHİN, Esra KOYUNCU, Mehseti BAYRAMOVA, Dursun KARASARTOVA
Ankara Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Ankara
Keywords: Salmonella serotype Typhimurium, plasmid profile, randomly amplified polimorphic DNA (RAPD), Turkey

The aim of this study was to investigate the characteristics of susceptible and multiresistant Salmonella serotype Typhimurium strains isolated in Turkey by plasmid profiles and randomly amplified polymorphic DNA (RAPD-PCR) patterns. Totally 55 S. Typhimurium clinical strains were selected from the culture collection of the Enterobacteria Laboratory of the Department of Microbiology and Clinical Microbiology, Medical School, Ankara University, for molecular analysis using plasmid profiles and the RAPD method. Forty-five isolates (82%) harbored one to three plasmids ranging in size from 1.0 to 100 kilobase pair (kbp). 90 kbp plasmids were the most common plasmids, and thirty-five strains (64%) carried 90kbp plasmids alone or together with other plasmids. Twenty strains (80%) of the isolates with AA/CCSSuT-R type carried the same plasmid profile: a single plasmid sized at 90 kbp. None of the strains analyzed displayed any RAPD-PCR bands with the primer OPB-17. By using primer p-1254, 46 strains (84%) were divided into 18 RAPD-PCR patterns. While the isolates with AA/CCSSuT-R type were divided into 7 RAPD patterns, 9 of these strains showed 100% similarity by the cluster analysis program. Analysis of RAPD-PCR with primer p-1254 can be used as an easy, rapid and discriminative method complementing antibiogram and plasmid profiles, and may contribute to the investigations of S. Typhimurium, which still cause outbreaks in Turkey. This study presents the first report on susceptible and multiresistant (including ones with AA/CCSSuT-R type) S. Typhimurium isolates in Turkey investigated by plasmid profiles and RAPD methods.

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