Candida species secrete various hydrolytic enzymes which are involved in infection pathogenesis. There are few studies on the hemolytic activity of Candida species. In this study, investigation of the hemolytic activity of the Candida species was aimed. Totally 70 Candida strains were investigated for in vitro hemolytic activity on Sabouraud dextrose agar containing sheep blood, supplied with 3% glucose. While 53 C. albicans and 2 C. guilliermondii strains exhibited alpha-beta hemolysis and 4 C. albicans strains revealed alpha hemolysis, no hemolytic activity was observed in 6 C. albicans, 3 C. kefyr, and 2 C. tropicalis strains. In conclusion, it was observed that Candida species exhibited variable hemolytic activity.