2009, Cilt 23, Sayı 1, Sayfa(lar) 009-012 |
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A MULTIPLEX POLYMERASE CHAIN REACTION ASSAY FOR THE DIAGNOSIS OF HERPESVIRUS INFECTIONS OF THE CENTRAL NERVOUS SYSTEM |
Hüseyin TAŞLI1, Murat SAYAN2 |
1Ege Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İzmir 2Kocaeli Üniversitesi Tıp Fakültesi Hastanesi Merkez Laboratuvarı, PCR Ünitesi, Kocaeli |
Keywords: Herpesviruses, central nervous system infections, cerebrospinal fluid, polymerase chain reaction |
Today, the polymerase chain reaction (PCR) analysis of the cerebrospinal fluid (CSF) is commonly used for the diagnosis of
herpesvirus infections of the central nervous system (CNS). Due to the great number of herpesviruses and limited amount of CSF,
PCR application offers limited throughput when viruses are screened individually. The purpose of this preliminary study was to
create and optimize a multiplex PCR (M-PCR) method that enables the simultaneous screening of five viruses including Herpes
simplex virus type 1 and type 2 (HSV-1, HSV 2), Varicella-zoster virus (VZV), Cytomegalovirus (CMV) and Epstein-Bar virus (EBV)
in CSF samples. Pairs of CSF samples, which were found positive for HSV-1, HSV-2, VZV and EBV but not for CMV by virusspecific
PCR reactions, were examined by M-PCR. In order to determine the presence of multiple viruses, CSF samples were mixed
and the examination was repeated. At the end of the study, expected virus DNAs could be determined in each sample and multiple
viruses could be determined in the mixed samples by M-PCR. Although this study shows that five herpesviruses can be easily
detected via a single amplification reaction, the method should be further evaluated by numerous clinical samples.
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