2009, Cilt 23, Sayı 2, Sayfa(lar) 083-087 |
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INVESTIGATION OF SHELF-LIVES OF MEDICAL MATERIALS USED IN OPERATING ROOMS AFTER STERILIZATION |
Sevim AKÇAĞLAR1, Sadık KILIÇTURGAY2, Beyza ENER1, Kamile ERCAN3, Okan TÖRE1 |
1Uludağ Üniversitesi, Tıp Fakültesi Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı, Bursa 2Uludağ Üniversitesi, Tıp Fakültesi Cerrahi Anabilim Dalı, Bursa 3Uludağ Üniversitesi, Sağlık Uygulama ve Araştırma Merkezi Sterilizasyon Ünitesi, Bursa |
Keywords: Sterilization, ethylene oxide, autoclave, shelf-life |
Sterilization refers to physical and chemical processes that effectively kill whole microorganisms such as bacteria and fungi
including spore forms and inactivate viruses. Effectiveness of various sterilization methods can be tested by different methods. The
quality of sterilization can differ according to storage conditions and packing qualities in different health centers. The aim of this
study was to determine the shelf-life of surgical equipment sterilized with different methods. Surgical equipment such as textile
products, plastic and metal tools that are packed with textile cloth, metal (unwrapped) and paper used in surgical wards and
operating rooms, at Uludag University Health Practice and Research Center, were investigated. These equipments were sterilized
with ethylene oxide and PPSS (Prevacuum Pressure Steam Sterilization- Autoclaving) and shelf-lives of the sterilized were weekly
and monthly investigated using microbiological methods. No microbiological growth was observed in materials packed with paper
and sterilized with ethylene oxide gas in weekly and monthly controls. Microbiological growth was only observed with PPSS
sterilization at the beginning of the 8th week. These growths were recorded in materials that are packed with paper and textile.
Stenotrophomonas malthophilia, Staphylococcus warnei and Staphylococcus hominis were detected in metal equipment packed with paper, plastic materials packed with paper and textile material packed with paper, respectively. No growth was observed in
materials packed in lidded metal boxes sterilized with PPSS. As a result of the study, it is suggested that ethylene oxide sterilization
of paper packages is a safe and long-life method and the most important factors affecting shelf-life of the materials are type of
package and method of sterilization.
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