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2009, Cilt 23, Sayı 4, Sayfa(lar) 179-183
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DIAGNOSIS OF AMOEBIASIS IN STOOL SAMPLES: COMPARISON OF NATIVE AND TRICHROME STAINED PREPARATIONS
Yasemin ZER1, İbrahim Halil KILIÇ2, Didem Işık KARAGÖZ2, Mehmet ÖZASLAN2, İlkay KARAOĞLAN3, İclal BALCI1
1Gaziantep Üniversitesi, Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Gaziantep
2Gaziantep Üniversitesi, Fen Fakültesi Biyoloji Bölümü, Gaziantep
3Gaziantep Üniversitesi, Tıp Fakültesi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Gaziantep
Keywords: Entamoeba histolytica/Entamoeba dispar, native method, trichrome staining, stool sample

Amoebiasis is a common parasitic infection caused by Entamoeba histolytica. The native method of stool samples is used frequently in the diagnosis of amoebiasis. Our aim was to compare native method and trichrome staining method in the diagnosis of this parasitic infection. Totally 215 stool samples, collected from children with diarrhoea, were investigated natively and stained with Gomori trichrome. In 97 stool samples examined with native method (45.1 %) amoeba cysts or vegetative forms were detected. After trichrome staining of these 97 stool samples E. coli was found in 75 (77.3 %) and E. histolytica/E. dispar in 11 (11.3 %); the parasite was not detected in 11 (11.3 %) samples. In addition, in 12 of 118 samples which were evaluated as negative with native technique E. coli was found by trichrome staining. We found false positive result in 40 % samples, but no false negative result. In conclusion, trichrome staining method is to be recommended in the diagnosis of amoebiasis.

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