Excessive use of vancomycin causes a decrease in susceptibility of staphylococci to this drug by means of treatment pressure. The aim of this study was to evaluate vancomycin resistance among Staphylococcus aureus strains isolated at a tertiary care hospital at which there is a high vancomycin consumption. One hundred and fourty-five isolates were screened by vancomycin (6 µg/ml) containing brain-heart infusion agar incubated for 24 and 48 hours. No vancomycin resistant S. aureus was detected. In spite of widespread use of vancomycin in the hospital fortunately no resistant S. aureus strain was detected.