The aim of this study was to investigate the presence of proteinase, phospholipase and slime activities which are important virulence factors in 100 Candida albicans strains isolated from blood, urine, vaginal discharge and oral cavity. The correlation between phosholipase and proteinase enzymes and the distribution of virulence factors of the isolates according to body parts were also studied. In 64 of 100 C. albicans isolates, proteinase activities were determined as one positive. Proteinase activity was were determined in 67.5% of the strains from vaginal discharge samples. Higher phosholipase activity was observed in vaginal discharge strains (Average Pz = 0.819 ± 0.12) and lower activity in oral cavity strains (Average Pz = 0.906 ± 0.094). The average phospholipase activity in all samples was 0.868 ± 0.112. Among the isolates, the high slime activity was determined in urine (52.5%) and blood (50%) samples. The correlation between proteinase and phospholipase activities were only observed in C. albicans strains from urine samples and for other clinical samples no correlation was determined. There was no correlation between the virulence factors and the parts of the body from which the strains were isolated. It is concluded that more than one virulence factor play role in the infections with C. albicans.