This study was undertaken to investigate the relation between the production of phospholipases and esterases and the development of oral candidosis. Twenty-five Candida albicans strains isolated from patients with oral candidosis and 29 strains from healthy individuals were included. The phospholipase activity of the isolates were evaluated with modified plate method. The esterase activity was examined by using Tween 80 opacity test. Twenty-two (88.0%) of the strains isolated from patients and 15 (51.7%) of the strains from healthy subjects were found to be phospholipase positive. A statistically significant difference was detected between the positivity rates of two groups (χ²=8.2; p=0.004). Nineteen (76.0%) isolates of the patients and 26 (89.7%) isolates from healthy subjects were found to be esterase positive after three days of incubation. No significant difference was observed between the groups (χ²=1.8; p=0.275). According to the results, it can concluded that phospholipase activity plays an important role in the pathogenesis of oral C. albicans infections while esterase activity does not seem to count as a virulence factor for these infections.