In this study clinical and laboratory features; isolated aerobic microorganisms and the antibiotic susceptibility test results of cases with diabetic foot infection were evaluated to make a contribution to the ampiric antibiotic treatment of such cases. Fifty cases were followed up prospectively.Thirtythree (66%) of cases were males and 17(34%) were females. According to Wagner classification seven cases were grade 1, 12 were grade 2, 13 were grade 3, 15 were grade 4 and three were grade 5. A total of 35 bacteria were isolated from 33(66%) cases; 14(40%) of them were gram-positive, 21(60%) were gram-negative. Staphyloccus aureus was the most common isolate among the gram-positive bacteria and the oxacillin resistance was detected in 37.5% of the strains. The most commonly isolated gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii. Induced beta-lactamase (IBL) was detected in one of the P. aerugnosa isolates and extended spectrum beta-lactamase (ESBL) was detected in one of the E. coli isolates. Imipenem/cilastatin was the only antibiotic to which all gram-negative bacteria were suscetible. Amputation was carried out in 32% of the cases.