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2007, Cilt 21, Sayı 3, Sayfa(lar) 129-133 |
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EVALUATION OF ELISA SEROLOGY WITH ANTI-HELICABACTER PYLORI IgG KIT FOR THE PRIMARY DIAGNOSIS OF HELICOBACTER PYLORI INFECTION IN DYSPEPTIC PATIENTS |
Yusuf ERZİN1, Mustafa ASLAN2, Ahmet DOBRUCALİ1, Sibel ALTUN2, Sibel ERDAMAR3, Ahmet DİRİCAN4, Bekir KOCAZEYBEK2 |
1İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Gastroenteroloji Bilim Dalı, İstanbul 2İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul 3İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul 4İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Biyoistatistik Anabilim Dalı, İstanbul |
Keywords: Helicobacter pylori, primary diagnosis, serology, ELISA |
Turkey is a country with a very high prevalence of Helicobacter pylori infection and diagnosis of the infection via invasive or
noninvasive tests is a major issue increasing the costs. The aim of this study was to assess the reliability of anti-H. pylori IgG ELISA
serology, a simple and inexpensive method, for the primary diagnosis of H. pylori infection in dyspeptic patients. Serum samples of
dyspeptic patients, who were referred to the endoscopy unit between June 2003 and March 2004 were tested with a commercially
available anti-H. pylori ELISA kit. Helicobacter pylori status of each patient was determined by histology, rapid urease test (RUT)
and culture. According to our gold standard, a patient was classified as being H. pylori-positive if the culture and/or both histology,
RUT were positive and as H. pylori-negative only if all of these tests remained negative. Patients with discordant results were
excluded. Standard methods were used to calculate sensitivity, specificity, predictive values of positive and negative results and
95% confidence intervals of these values. A total of 152 patients were studied. According to our defined gold standard, 131 patients
(86%) were H. pylori-positive and 21 patients (14%) were H. pylori-negative. The sensitivity, specificity and diagnostic accuracy of
the IgG-ELISA were 97%, 67% and 93%, respectively. Due to its lack for specificity, it is concluded that anti-H. pylori ELISA test
may not be a suitable alternative for the primary diagnosis of H. pylori infection in dyspepsia patients.
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