In microbiologic documentation, microscopic images are stored in electronic logical devices or printed materials. In each way, unwanted artefacts occur due to physical limitations of refraction in optic systems. Routine reproduction devices have much lower dydynamic range than the radiance maps (or equivalently real work scenes). In this study, evaluation of high dynamic range (HDR) scenes created by digital camera applied to light microscopy for more presentable mycological microscopic images was mode. Candida dubliniensis and Dreschlera sp. were subcultured at room temperature on 1% Tween-80 supplemented cornmeal agar with surface coverslip and potato dextrose agar plate, respectively. At the and of 72-hour incubation, consecutive microscopic images were taken from slides by direct cornmeal agar surface for chlamydospore and hyphal formation. Macroconidia and related hyphal structure images were taken by pressed cellotape slide method from potato dextrose agar surface. Remote shooting personal digital camera was adapted to light microscope. HDR images were created by open-source software with continuous scenes from -2 to +2 exposure range step by +1 interval. All parameters directly effecting images such as microscopic light source nature, were tested for higher quality. Several tone mapping curves and operators by open-source program were applied. As a result, HDR images are more useful than radiance maps (or low dynamic range photographs) for lower magnification microscopic images in mycology documentation. HDR images without tone mapping may not be more presentable than directly images. Different tone mapping procedures into HDR images are useful for presenting fungal structure. Present digital cameras are sufficient for HDR images in microscopy.