2008, Cilt 22, Sayı 4, Sayfa(lar) 203-207 |
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INVESTIGATION OF PLASMID MEDIATED HIGH LEVEL AMINOGLYCOSIDE RESISTANCE IN GRAM-NEGATIVE BACTERIA |
Şafak ERMERTCAN1, Mine HOŞGÖR-LİMONCU1, Hüseyin TAŞLI1, Bayrı ERAÇ1, Hörü GAZİ2 |
1 Ege Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İzmir 2 Celal Bayar Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Manisa |
Keywords: Gram-negative bacteria, R-plasmid, high level aminoglycoside resistance |
Gram-negative bacteria are the most important infective agents in hospitalized patients. Aminoglycosides are still important in the
treatment of infections caused by Gram-negative bacteria. In recent years a new resistance mechanism emerging by methylation of
the bacterial 16S rRNA has been reported. This mechanism, causing high-level aminoglycoside resistance, is coded on plasmids
and easily transferred among Gram-negative bacteria. The purpose of this study was to investigate aminoglycoside resistance
mechanism acquired by 16S rRNA methylase enzymes in Gram-negative bacteria. With microdilution method high-level
aminoglycoside resistance was detected only in one Acinetobacter baumannii strain out of 112 Gram-negative bacteria. Methylases
enzymes genes were not observed by polymerase chain reaction+agarose gel electrophoresis in this strain. 16S rRNA methylase
genes are usually found with beta-lactamase genes and confer high-level resistance against most of the aminoglycosides.
Therefore, investigation of this resistance mechanism by further studies in our country is required.
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