2009, Cilt 23, Sayı 2, Sayfa(lar) 051-055 |
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INVESTIGATION OF METALLO-BETA-LACTAMASE PRODUCTION IN ACINETOBACTER BAUMANII AND PSEUDOMONAS AERUGINOSA ISOLATES WITH FOUR DIFFERENT PHENOTYPIC METHODS |
Emel SESLİ-ÇETİN1, Tülay TETİK2, Selçuk KAYA1, Buket CİCİOĞLU-ARIDOĞAN1 |
1Süleyman Demirel Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Isparta 2Sivas Devlet Hastanesi Mikrobiyoloji Laboratuvarı, Sivas |
Keywords: Pseudomonas aeruginosa, Acinetobacter baumannii, metallo-beta-lactamase, phenotypic methods |
The aim of this study was to determine the prevalance of metallo-beta-lactamase (MBL) production among Acinetobacter baumannii
and Pseudomonas aeruginosa isolates which were isolated from clinical specimens in the Microbiology Laboratory of Suleyman
Demirel University Medical Faculty between January and December 2007. The study was done with a total of 330 isolates, 189
P. aeruginosa and 141 A. baumannii strains. Sixty-one (43.3%) A. baumannii and 52 (27.5%) P. aeruginosa isolates which were
determined as resistant to imipenem were tested for the presence of MBL production by using combined disk, double disk synergy,
modified Hodge and MBL-E tests. Among imipenem resistant A. baumannii and P. aeruginosa strains, the MBL production rate was
found as 84% and 73% by IPM-EDTA double disk synergy test, 75% and 62% by combined disc method, 74% and 58% by modified
Hodge test and as 80%, 40% by MBL E test, respectively. Of A. baumannii strains 52.5% and of P. aeruginosa strains 21.2% were
detected as MBL producers by all of the four different phenotypic methods. In conclusion, the MBL positivity rates determined
among A. baumannii and P. aeruginosa isolates of our hospital were considerably high. It is concluded that early detection of MBLproducing
A. baumannii and P. aeruginosa isolates is of great importance in the on-time control of nosocomial infections.
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