The aim of this study was to determine the rates of Mycobacterium tuberculosis complex and their resistance to antituberculous agents from the specimens accepted to the Microbiology Laboratory, Trakya University Hospital, between November 1999 and November 2001. In the study, also the isolation rates of nontuberculous mycobacteria were investigated. For this purpose, 3816 specimens were examined using BACTEC 460 method and M. tuberculosis complex was identified in 134 (96.4%) of them and nontuberculous mycobacteria were identified in five (3.6%). Resistance rates of M. tuberculosis complex isolates were 9%, 4.5%, 2.2% and 1.5% for isoniazid, rifampin, streptomycin and ethambutol, respectively. Resistant rate to at least one agent was 6%, and to both isoniazid and rifampin was 3%. The number of the isolates sensitive to four major antituberculous agents was 119 (88.8%). The results obtained were compared with the results of the previous studies in the Trakya Region and Turkey.