The purpose of this study was to assess the antituberculosis potential of some drugs including metronidazole, fluconazole, econazole, miconazole, clotrimazole, ketoconazole, amphotericin B, 5-fluorocytosine in comparison to well-known primary and secondary (flouroquinolones) antituberculosis agents. The MIC values were determined with broth microdilution method. The MIC values for Mycobacterium tuberculosis H37Rv of isoniazid, rifampicin, streptomycin and ethambutol (primary antituberculosis agents) were 0.125 µg/ml, <0.06 µg/ml, µ2 g/ml and 4 g/ml, respectively. Ofloxacin, ciprofloxacin, pefloxacin, metronidazole, clotrimazole, and ketoconazole of MIC values were 0.5 µg/ml, 1 µg/ml, 4 µg/ml, >256 µg/ml, 16 µg/ml and 16 µg/ml, respectively. The results of the study showed that some antimicrobial agents particularly clotrimazole and ketoconazole have antituberculosis activity and they should be studied further to determine their in vivo effect in tuberculosis.