The currently used vaccine strain Mycobacterium bovis BCG, may cause disseminated infection in patients with congenital or acquired immune deficiency. Disseminated BCG infection developed shortly after BCG vaccination in a 9 month-old of male baby with homozygous IFN-γ receptor deficiency. The acid-fast staining of the dissected axillary lymph node revealed numerous acid-fast bacilli. Which later grew on MB/BacT (bioMérieux, Marcy l'Etoile, France) automated system and Löwenstein-Jensen slant after 31.5 day and 4 week incubation periods, respectively. The culture growing bacteria was identified as M. bovis BCG by Genotype MTBC assay (Hain Lifescience, Nehren, Germany) based on PCR-reverse hybridization.