Brucellosis is a common worldwide public health problem which affect different organs. Because of the high rate of relapse in brucellosis, new treatment strategies are needed. The aim of this study was to compare tigecycline, a new antibiotic, with other antibiotics the effects of which on brucellosis are well known. No data exists regarding this issue. The present study consisted of 82 Brucella species isolated from cultures of blood or bone marrow. The sensitivity of all isolates against tigecycline, doxycycline, ciprofloxacin, and rifampin were tested using E-test method. It was found that minimal inhibitor concentration (MIC90) of doxycycline was lower whereas (MIC90) of rifampin was higher. While all isolates were susceptible to ciprofloxacin and doxycycline, intermediate resistance to rifampin was detected in 19 (23%) isolates. Although the present study showed that in vitro tigecycline was more effective than doxycycline on Brucella species, in vivo studies are needed to determine the effecacy of this drug in the treatment of brucellosis.