The aim of this study was to determine the antibiotic suvceptibility, 814 S. aureus strains isolated from various clinical specimens in the Bacteriology Laboratory, Celal Bayar Univesity Hospital, Manisa, Turkia, 1998-2002. The antibiotic susceptibilities of the strains to methicillin, vancomycin, teicoplanin, erythromycin, tetracyline, gentamicin, clindamycin, ciprofloxacin, rifampin and trimethoprimsulfamethoxazole were investigated by disk diffusion method according to the recommendations of the NCCLS. Of the 814 S. aureus strains, 264 (32.4%) were methicillin resistant (MRSA) and 550 (67.6%) were methicillin susceptible (MSSA). The resistance rates of MRSA and MSSA strains were found as follows, respectively erythromycin 59.5% and 14.2%, tetracyline 57.6% and 23.5%, gentamicin 28.4% and 8.4%, clindamycin: for 28.4% and 10.2%, ciprofloxasin 34.1% and 14.7%, rifampin 31.4% and 16.0%, TMP/SMX 23.1% and 6.9%. All strains were sensitive to vancomycin and teicoplanin.