In recent years with the investigation of HBV DNA isolated from patients showing anti-HBe seroconversion without the loss of viral replication revealed precore/core gene mutations. In this study, we aimed at investigating the relationship between precore/core mutations and HBV genotype profile in anti-HBe and HBV DNA positive patients. In the sera of these patients HBV DNA levels were investigated by Real-Time PCR method. In the HBV DNA positive specimens precore/core mutations were detected by sequence analysis. Of the 100 anti-HBe positive chronic hepatitis B patients' serum samples HBV DNA was found positive in 51. The median HBV DNA viral load was 4.5x10⁷ copies/mL in HBV DNA positive specimens. All of the HBV (100%) genotyped was found in genotype D. There were no precore/core mutations by using DNA Sequencing Analysis Software. These results show that all patients investigated in this study were infected with wild-type HBV and the viral loads obtained belonged to the wild-type HB virus. Multicenter studies with more cases are needed to investigate the precore/core mutations which play an important role in the replication rate of HBV and in the control of the HBe production in chronic HBV infections.