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2006, Cilt 20, Sayı 2, Sayfa(lar) 117-120
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DETERMINATION OF INDEX VALUES TO PREVENT REPETITIONS IN BLOOD DONOR SCREENING ASSAYS BY AxSYM
Aydan ÖZKÜTÜK1, Alp ERGÖR2, Melike GÜZÜNLER1, A. Arzu SAYINER1, Y. Hakan ABACIOĞLU1
1Dokuz Eylül Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İzmir
2Dokuz Eylül Üniversitesi Tıp Fakültesi, Halk Sağlığı Anabilim Dalı, İzmir
Keywords: Blood donor, enzyme immunoassay, ROC analysis, sample/cut off value

The microparticle-based enzyme-linked immunoassay (AxSYM; Abbott Laboratories) can simultaneously detect antibodies against Human Immunodeficiency Virus (anti-HIV), Hepatitis C Virus (anti-HCV) and Hepatitis B Virus surface antigen (HBsAg) which are widely used for screening of blood donors for HIV, HBV, HCV infections. Nevertheless, reactivity on a screening immunoassay may represent false reactivity, particularly in low-risk populations such as voluntary blood donors. Therefore, some supplemental tests such as immunoblot and/or nucleic acid determination assays are required for repeatedly reactive samples. This study was planned to determine the EIA index value (s/co) that can differentiate the repeatedly reactive samples from false reactive ones in order to decrease the need for EIA repetitions. Sera of 196 blood donors were tested for anti-HCV, anti-HIV and HBsAg in order to determine the EIA indexes of the repeatedly reactive samples. The threshold EIA indexes of three assays with highest sensitivity and specificity were determined by using receiver operating characteristics (ROC) analysis. These values were ≥ 2.28 for anti-HCV, ≥ 5.22 for HBsAg and ≥2.78 for anti-HIV. Samples with EIA indexes above these values may be accepted as repeatedly reactive and further EIA repetitions can be avoided before performing supplemental tests.

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