The close relationship between Cryptococcus neoformans and natural flora shows that decaying wood inside tree trunk hollows is a natural source of C. neoformans in urban regions. In this study, many monumental trees from 11 different provinces in Anatolia, Turkey, were investigated for the presence of C. neoformans using Guizotia abyssinica agar containing biphenyl for isolation. No C. neoformans was isolated from monumental trees. Natural flora might be reservoir for the yeasts which are strongly dependent on environment chemistry. The alkaline property of Anatolian soil may constitute a barrier for the spread and colonization of the yeast.