An essential component of the fungal cell wall is chitin (C₈H₁₃O₅N)_n which is disributed in infected tissues. Quantitative determination of the chitin shows the fungal burden in the tissues. The purpose of this study was to determine the chitin levels in tissues of animal models with aspergillosis and candidiosis, with the comparison of these levels with other infection signs. The presence of the infection was confirmed by using culture and pathology in tissue sections whereas RealTime – PCR in blood samples. The quantification of the chitin was done spectrophotometrically. Mean optical dansities of the tissue extracts with aspergillosis was 0.373 while it was 0.492 with candidosis. The difference was statistically significant (p<0.05). As a result of our study, it was found that chitin is less distributed in the tissues with aspergillosis than those with candidosis. Otherwise, the presence of chitin was found to be well correlated with culture and pathology results in candidosis and aspergillosis. But the presence of chitin was found to be well correlated with PCR results in candidosis, but not in aspergillosis.