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2008, Cilt 22, Sayı 3, Sayfa(lar) 147-151 |
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EVALUATION OF ELISA SEROLOGY WITH ANTI-HELICOBACTER PYLORI FOR THE PRIMARY IgG KIT DIAGNOSIS OF HELICOBACTER PYLORI INFECTION IN DYSPEPTIC PATIENTS |
Yusuf ERZİN1, Sibel ALTUN2, Ahmet DOBRUCALİ1, Mustafa ASLAN2, Sibel ERDAMAR3, Ahmet DİRİCAN4, Bekir KOCAZEYBEK2 |
1 İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Gastroenteroloji Bilim Dalı, İstanbul 2 İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul 3 İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul 4 İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Biyoistatistik Anabilim Dalı, İstanbul |
Keywords: Helicobacter pylori, primary diagnosis, serology, ELISA |
Turkey is a country with a very high prevalence of Helicobacter pylori infection and diagnosis of the infection via invasive or
noninvasive tests is a major issue increasing the costs. The aim of this study was to assess the reliability of anti-H. pylori IgG ELISA
serology, a simple and inexpensive method, for the primary diagnosis of H. pylori infection in dyspeptic patients. Serum samples of
dyspeptic patients, who were referred to the Endoscopy Unit, Cerrahpaşa Medical School, İstanbul, between June 2003 and March
2004, were tested with a commercially available anti-H. pylori ELISA kit. Helicobacter pylori status of each patient was determined
by histology, rapid urease test (RUT) and culture. According to our gold standard, a patient was classified as being H. pylori-positive
if the culture and/or both histology, RUT were positive and as H. pylori-negative only if all of these tests remained negative. Patients
with discordant results were excluded. Standard methods were used to calculate sensitivity, specificity, predictive values of positive and negative results and 95% confidence intervals of these values. Totally 152 patients were studied. According to our defined gold
standard, 131 patients (86%) were H. pylori-positive and 21 patients (14%) were H. pylori-negative. The sensitivity, specificity and
diagnostic accuracy of the IgG-ELISA were 97%, 67% and 93%, respectively. Due to its lack for specificity, it is concluded that anti-
H. pylori ELISA test may not be a suitable alternative for the primary diagnosis of H. pylori infection in dyspepsia patients.
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